Thermolysin in human cultured keratinocyte isolation
نویسندگان
چکیده
منابع مشابه
Thermolysin in human cultured keratinocyte isolation.
BACKGROUND When treating extensively burned patients using cultured epidermal sheets, the main problem is the time required for its production. Conventional keratinocyte isolation is usually done using Trypsin. We used a modification of the conventional isolation method in order to improve this process and increase the number of colonies from the isolated epidermal cell population. PURPOSE To...
متن کاملIsolation and Cultivation of Adult Human Keratinocyte Stem Cells for Regeneration of Epidermal Sheets
Background: Keratinocyte stem cell is one of the adult stem cells that inhabits the skin and contributes to skin function and renewal. Adult stem cells are best defined by their capacity to self-renew, and to maintain tissue function for a long period of time. These findings indicate the importance of these cells for clinical applications including regenerative medicine, tissue engineering and ...
متن کاملIsolation of human oral keratinocyte progenitor/stem cells.
Progenitor/stem cell populations of epithelium are known to reside in the small-sized cell population. Our objective was to physically isolate and characterize an oral keratinocyte-enriched population of small-sized progenitor/stem cells. Primary human oral mucosal keratinocytes cultured in a chemically defined serum-free culture system, devoid of animal-derived feeder cells, were sorted by rel...
متن کاملHuman beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients.
PURPOSE To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by dermic fibroblasts and keratinocytes cultivated from burned patients' skin samples. METHODS Keratinocytes and fibroblasts of 10 patients (four major burns, four minor burns and two controls) were primarily cultivated according to standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR. RESULTS In fi...
متن کاملisolation and cultivation of adult human keratinocyte stem cells for regeneration of epidermal sheets
background: keratinocyte stem cell is one of the adult stem cells that inhabits the skin and contributes to skin function and renewal. adult stem cells are best defined by their capacity to self-renew, and to maintain tissue function for a long period of time. these findings indicate the importance of these cells for clinical applications including regenerative medicine, tissue engineering and ...
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ژورنال
عنوان ژورنال: Brazilian Journal of Biology
سال: 2007
ISSN: 1519-6984
DOI: 10.1590/s1519-69842007000100014